Simultaneous identification of multiple mammalian species from mixed forensic samples based on mtDNA control region length polymorphism

被引：10

作者：

Fumagalli, Luca ^{[1
,2
,3
]}

Cabrita, Catherine Jan ^{[1
,2
]}

Castella, Vincent ^{[1
,2
]}

机构：

[1] CHU Vaudois, Ctr Univ Romand Med Lekale, Unite Genet Forens, CH-1011 Lausanne, Switzerland

[2] Univ Lausanne, CH-1011 Lausanne, Switzerland

[3] Univ Lausanne, Dept Ecol & Evolut Biophore, Lab Biol Conservat, CH-1015 Lausanne, Switzerland

来源：

FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES | 2009年 / 2卷 / 01期

关键词：

DNA mixtures; Species identification; Wildlife forensics; Mitochondrial DNA control region;

D O I：

10.1016/j.fsigss.2009.08.009

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Molecular species identification in mixed or contaminated biological material has always been problematic. We developed a simple and accurate method for mammal DNA identification in mixtures, based on interspecific mitochondrial DNA control region length polymorphism. Contrary to other published methods dealing with species mixtures, our protocol requires a single universal primer pair and amplification step, and is not based on a pre-defined panel of species. This protocol has been routinely employed by our laboratory for species identification in dozens of human and animal forensic caseworks. Six representative forensic caseworks involving the specific identification of mixed animal samples are reported in this paper, in order to demonstrate the applicability and usefulness of the method. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：302 / 303

页数：2

共 4 条

[1]

Pun K.-M., Albrecht C., Castella V., Fumagalli L., Species identification in mammals from mixed biological samples based on mitochondrial DNA control region length polymorphism, Electrophoresis, 30, pp. 1008-1014, (2009)

[2]

Taberlet P., Bouvet J., Mitochondrial DNA polymorphism, phylogeography, and conservation genetics of the brown bear Ursus arctos in Europe, Proc. R. Soc. Lond. B, 255, pp. 195-200, (1994)

[3]

Fumagalli L., Taberlet P., Favre L., Hausser J., Origin and evolution of homologous repeated sequences in the mitochondrial DNA control region of shrews, Mol. Biol. Evol., 13, pp. 31-46, (1996)

[4]

Valiere N., Fumagalli L., Gielly L., Miquel C., Lequette B., Poulle M.L., Weber J.M., Arlettaz R., Taberlet P., Long-distance wolf recolonization of France and Switzerland inferred from non-invasive genetic sampling over a period of 10 years, Anim. Cons., 6, pp. 83-92, (2003)

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