DEVELOPMENT OF A MODIFIED MICRONUCLEUS ASSAY INVITRO FOR DETECTION OF ANEUGENIC EFFECTS

被引:15
作者
SEELBACH, A
FISSLER, B
STROHBUSCH, A
MADLE, S
机构
[1] Max-von-Pettenkofer-Institute, Federal Health Office, D-1000 Berlin 33
关键词
D O I
10.1016/0887-2333(93)90130-W
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
A simple and rapid micronucleus (MN) assay in vitro has been developed with V79 cells enabling preferential detection of aneugen-induced MN. V79 cells were treated with test compounds for 3 hr, the mitoses were shaken off and transferred into new flasks, and after a 3.5-hr recovery period interphase cells were analysed for MN. Using this time schedule only cells that were treated during mitosis or G2-phase plus mitosis were analysed. Four suspected aneuploidy-inducing agents (aneugens) were tested: griseofulvin, methyl 2-benzimidazole carbamate, diazepam and thiobendazole. All four compounds induced MN in the modified V79 assay. For further characterization of the modified V79/MN assay the clastogen mitomycin C (MMC) was investigated with respect to the time-dependency of MN induction. MMC did not induce MN in our modified V79 assay, but was positive after 14 hr of exposure. Induced MN were characterized by CREST staining and by measuring their size. The frequencies of CREST-positive and large MN were increased by the four suspected aneugens analysed. Furthermore, the use of the external metabolization systems, S-9 mix, and hepatocytes, was examined.
引用
收藏
页码:185 / 193
页数:9
相关论文
共 30 条
[1]   METHODS FOR DETECTING CARCINOGENS AND MUTAGENS WITH SALMONELLA-MAMMALIAN-MICROSOME MUTAGENICITY TEST [J].
AMES, BN ;
MCCANN, J ;
YAMASAKI, E .
MUTATION RESEARCH, 1975, 31 (06) :347-363
[2]   DIAZEPAM INDUCES MITOTIC ARREST AT PROMETAPHASE BY INHIBITING CENTRIOLAR SEPARATION [J].
ANDERSSON, LC ;
LEHTO, VP ;
STENMAN, S ;
BADLEY, RA ;
VIRTANEN, I .
NATURE, 1981, 291 (5812) :247-248
[3]   INVITRO MICRONUCLEUS TEST WITH KINETOCHORE STAINING - EVALUATION OF TEST-PERFORMANCE [J].
ANTOCCIA, A ;
DEGRASSI, F ;
BATTISTONI, A ;
CILIUTTI, P ;
TANZARELLA, C .
MUTAGENESIS, 1991, 6 (04) :319-324
[4]   MUTAGENICITY OF METHYL 2-BENZIMIDAZOLECARBAMATE, DIETHYLSTILBESTROL AND ESTRADIOL - STRUCTURAL CHROMOSOMAL-ABERRATIONS, SISTER-CHROMATID EXCHANGES, C-MITOSES, POLYPLOIDIES AND MICRONUCLEI [J].
BANDUHN, N ;
OBE, G .
MUTATION RESEARCH, 1985, 156 (03) :199-218
[5]   NEW CULTURE MODEL FACILITATING RAPID QUANTITATIVE TESTING OF MITOTIC SPINDLE INHIBITION IN MAMMALIAN-CELLS [J].
DEBRABANDER, M ;
VANDEVEIRE, R ;
AERTS, F ;
GEUENS, S ;
HOEBEKE, J .
JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1976, 56 (02) :357-363
[6]   GRISEOFULVIN [J].
DECARLI, L ;
LARIZZA, L .
MUTATION RESEARCH, 1988, 195 (02) :91-126
[7]   IMMUNOFLUORESCENT STAINING OF KINETOCHORES IN MICRONUCLEI - A NEW ASSAY FOR THE DETECTION OF ANEUPLOIDY [J].
DEGRASSI, F ;
TANZARELLA, C .
MUTATION RESEARCH, 1988, 203 (05) :339-345
[8]  
DEPARGAMENT MDM, 1987, MUTAT RES, V188, P1
[9]  
DUSTIN P, 1984, MICROTUBULES
[10]   KINETOCHORE LOCALIZATION IN MICRONUCLEATED CYTOKINESIS-BLOCKED CHINESE-HAMSTER OVARY CELLS - A NEW AND RAPID ASSAY FOR IDENTIFYING ANEUPLOIDY-INDUCING AGENTS [J].
EASTMOND, DA ;
TUCKER, JD .
MUTATION RESEARCH, 1989, 224 (04) :517-525