PURIFICATION AND CHARACTERIZATION OF LIPID BIOFLOCCULANT PRODUCED BY RHODOCOCCUS-ERYTHROPOLIS

被引：44

作者：

KURANE, R

HATAMOCHI, K

KAKUNO, T

KIYOHARA, M

KAWAGUCHI, K

MIZUNO, Y

HIRANO, M

TANIGUCHI, Y

机构：

[1] KANSAI ELECT POWER CO, AMAGASAKI, HYOGO 661, JAPAN

[2] TORAY RES CTR LTD, KAMAKURA, KANAGAWA 248, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 11期

关键词：

D O I：

10.1271/bbb.58.1977

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The bioflocculant produced by Rhodococcus erythropolis S-1 was found to exist as huge assemblies, the molecular mass of which is over one million daltons, composed of many polypeptides and lipids in aqueous solution. We have isolated and purified this lipid bioflocculant by ultracentrifugation, extracting with 90% acetone, and two successive silica gel chromatographies from the culture broth. It was homogeneous on silica gel thin-layer chromatography. H-1-NMR and HPLC studies showed that it was a kind of glycolipid that contained a C-16 methylene chain on the average and glucose in its chemical structure. The flocculating activity against kaolin clay suspension was dependent on the Ca2+ concentration.

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页码：1977 / 1982

页数：6

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