CHARACTERIZATION OF A NOVEL MUCIN SULFOTRANSFERASE ACTIVITY SYNTHESIZING SULFATED O-GLYCAN CORE 1,3-SULFATE-GAL-BETA-1-3GALNAC-ALPHA-R

被引：39

作者：

KUHNS, W

JAIN, RK

MATTA, KL

PAULSEN, H

BAKER, MA

GEYER, R

BROCKHAUSEN, I

机构：

[1] HOSP SICK CHILDREN, RES INST, TORONTO, ON M5G 1X8, CANADA

[2] ROSWELL PK MEM INST, BUFFALO, NY USA

[3] UNIV HAMBURG, INST ORGAN CHEM, W-2000 HAMBURG, GERMANY

[4] TORONTO GEN HOSP, DEPT MED, TORONTO, ON M5G 1L7, CANADA

[5] UNIV GIESSEN, INST BIOCHEM, W-6300 GIESSEN, GERMANY

[6] UNIV TORONTO, DEPT BIOCHEM, TORONTO, ON, CANADA

来源：

GLYCOBIOLOGY | 1995年 / 5卷 / 07期

关键词：

COLONIC TISSUE; O-GLYCANS; MUCIN; MUCIN BIOSYNTHESIS; SULFOTRANSFERASE;

D O I：

10.1093/glycob/5.7.689

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel sulphotransferase (sulpho-T) activity from rat colonic mucosa was characterized using O-glycan core 1 substrate, Gal beta 1-3GalNAc alpha-benzyl. Derivatives of Gal beta 1-3GaINAc- were used to demonstrate that the 3- and 4-hydroxyl of Gal and the 2-acetamido group of the GalNAc residue of Gal beta 1-3GalNAc alpha-benzyl substrates were important for activity, Sulphated product using Gal beta 1,3GalNAc alpha-benzyl as substrate was analysed by ion spray mass spectrometry, methylation analysis, high-pH anion-exchange chromatography and beta-galactosidase digestion, The results suggested that sulphate was added to the 3-position of the Gal residue, The synthesis of core 2 from core 1 by UDP-GlcNAc: Gal beta 1-3GalNAc beta 6-GlcNAc-transferase was inhibited by sulphation of the Gal residue, indicating that GlcNAc beta 1-6 branching has to precede sulphation in the O-glycan core 1 processing pathway. These data demonstrate several novel pathways in the synthesis of sulphated mucin-type oligosaccharides.

引用

页码：689 / 697

页数：9

共 62 条

[1] SYNTHETIC STUDIES IN CARBOHYDRATES .25. SYNTHESIS OF BENZYL 2-ACETAMIDO-3-O AND 6-O-(2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOPYRANOSYL)-2-DEOXY-ALPHA-D-GALACTOPYRANOSIDE [J].