CHARACTERIZATION OF A NOVEL MUCIN SULFOTRANSFERASE ACTIVITY SYNTHESIZING SULFATED O-GLYCAN CORE 1,3-SULFATE-GAL-BETA-1-3GALNAC-ALPHA-R

被引:39
作者
KUHNS, W
JAIN, RK
MATTA, KL
PAULSEN, H
BAKER, MA
GEYER, R
BROCKHAUSEN, I
机构
[1] HOSP SICK CHILDREN, RES INST, TORONTO, ON M5G 1X8, CANADA
[2] ROSWELL PK MEM INST, BUFFALO, NY USA
[3] UNIV HAMBURG, INST ORGAN CHEM, W-2000 HAMBURG, GERMANY
[4] TORONTO GEN HOSP, DEPT MED, TORONTO, ON M5G 1L7, CANADA
[5] UNIV GIESSEN, INST BIOCHEM, W-6300 GIESSEN, GERMANY
[6] UNIV TORONTO, DEPT BIOCHEM, TORONTO, ON, CANADA
关键词
COLONIC TISSUE; O-GLYCANS; MUCIN; MUCIN BIOSYNTHESIS; SULFOTRANSFERASE;
D O I
10.1093/glycob/5.7.689
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A novel sulphotransferase (sulpho-T) activity from rat colonic mucosa was characterized using O-glycan core 1 substrate, Gal beta 1-3GalNAc alpha-benzyl. Derivatives of Gal beta 1-3GaINAc- were used to demonstrate that the 3- and 4-hydroxyl of Gal and the 2-acetamido group of the GalNAc residue of Gal beta 1-3GalNAc alpha-benzyl substrates were important for activity, Sulphated product using Gal beta 1,3GalNAc alpha-benzyl as substrate was analysed by ion spray mass spectrometry, methylation analysis, high-pH anion-exchange chromatography and beta-galactosidase digestion, The results suggested that sulphate was added to the 3-position of the Gal residue, The synthesis of core 2 from core 1 by UDP-GlcNAc: Gal beta 1-3GalNAc beta 6-GlcNAc-transferase was inhibited by sulphation of the Gal residue, indicating that GlcNAc beta 1-6 branching has to precede sulphation in the O-glycan core 1 processing pathway. These data demonstrate several novel pathways in the synthesis of sulphated mucin-type oligosaccharides.
引用
收藏
页码:689 / 697
页数:9
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