LACK OF ESTRADIOL-DNA BINDING IN MOUSE EMBRYO CELL-CULTURES OR FOLLOWING RAT-LIVER MICROSOMAL METABOLISM

Oestradiol-17β was compared to benzo[a]pyrene (BP) for its ability to bind to the DNA of mouse embryo cells in culture or to DNA added to a rat liver microsomal incubation mixture. No significant binding was found in embryo cells (at least 100-fold less than for BP). Microsomal incubation resulted in apparent oestradiol binding to a reisolated DNA-containing complex, but most of this was lost when the DNA was freed of RNA and protein. © 1979 Elsevier/North-Holland Scientific Publishers Ltd., All rights reserved.