CHARACTERIZATION OF A MONOCLONAL-ANTIBODY WHICH IS AN ACTIVATOR OF RABBIT PLATELETS

被引:1
作者
CHAU, LY
LIN, JR
机构
[1] Institute of Biomedical Sciences, Academia Sinica, Nankang, Taipei
关键词
MONOCLONAL ANTIBODY; PLATELET; ACTIVATION;
D O I
10.1016/0304-4165(91)90049-M
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A monoclonal antibody (MAb) raised against rabbit platelet membranes was shown to be a strong agonist to induce platelet aggregation and secretion. This MAb, designated 19CB-1, was identified as an IgM and purified to near homogeneity by ammonium sulfate precipitation and Q-sepharose column chromatography. Aggregation induced by 19CB-1 was only slightly affected in the presence of creatine phosphate / creatine phosphokinase and aspirin, indicating that it was not mediated through the cyclooxygenase pathway and the release of ADP. 19CB-1 Fab fragments did not induce platelet aggregation. However, 19CB-1-induced aggregation was inhibited by these Fab fragments. 19CB-1 also elicited a rise in cytoplasmic calcium concentration in fura-2 loaded platelets. In the absence of external calcium, a substantial calcium signal remained to be observed, suggesting the release of calcium from intracellular stores in response to 19CB-1. This MAb reacted primarily with a polypeptide of M(r) = 57 000, as revealed by immunoblotting. These results suggest that the 57 kDa antigen is one of the platelet surface proteins directly involved in the activation of rabbit platelets.
引用
收藏
页码:118 / 124
页数:7
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