RIBOSOMAL DNA-SEQUENCES IN THE DIFFERENTIATION OF PATHOGENIC AND NONPATHOGENIC ISOLATES OF ENTAMOEBA-HISTOLYTICA

被引：34

作者：

CRUZREYES, JA

SPICE, WM

REHMAN, T

GISBORNE, E

ACKERS, JP

机构：

[1] Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, London, Keppel Street

来源：

PARASITOLOGY | 1992年 / 104卷

基金：

英国惠康基金;

关键词：

ENTAMOEBA-HISTOLYTICA; RIBOSOMAL GENES; RFLPS;

D O I：

10.1017/S0031182000061679

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Recombinant ribosomal DNA sequences were amplified by PCR and used as probes to perform a fingerprint analysis of total DNA from different Entamoeba histolytica isolates. RFLPs obtained with one of the probes, R-1, support previous proposals that pathogenic and non-pathogenic E. histolytica are closely related, yet genotypically distinct. Another probe, R-2, while not distinguishing between the two forms of E. hystolytica, was able to differentiate between them and E. moshkovskii, which has morphologically identical cysts and trophozoites. A third probe, BR-1, identified strain-specific RFLPs.

引用

页码：239 / 246

页数：8

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