ACOUSTIC PLATE MODE SENSOR FOR IMMUNOCHEMICAL REACTIONS

被引：35

作者：

DAHINT, R

GRUNZE, M

JOSSE, F

RENKEN, J

机构：

[1] UNIV HEIDELBERG,LEHRSTUHL ANGEW PHYS CHEM,D-69120 HEIDELBERG,GERMANY

[2] MARQUETTE UNIV,DEPT ELECT & COMP ENGN,MILWAUKEE,WI 53233

来源：

ANALYTICAL CHEMISTRY | 1994年 / 66卷 / 18期

关键词：

D O I：

10.1021/ac00090a015

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A practical immunosensor, based on a 150-MHz acoustic plate mode (APM) device on ZX-LiNbO3 has been built and is employed to monitor directly, in real time, antigen-antibody reactions via mass loading of the crystal surface. The results show frequency shifts similar to 1 kHz above the noise level upon antigen adsorption. The mass density detection limit of our present sensor is similar to 200 pg/mm(2), corresponding to 8.10(8) molecules/mm(2) utilizing a typical Ige of 150 000 Da. Antigen binding capacities of aminosilane films are measured independently by a modified ELISA test and are related to the APM sensor response.

引用

页码：2888 / 2892

页数：5

共 12 条

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