IDENTIFICATION OF GAMMA-LIKE DNA-POLYMERASE FROM SEA-URCHIN EMBRYOS

被引:19
作者
HABARA, A
NAGANO, H
MANO, Y
机构
[1] Department of Physiological Chemistry and Nutrition, Faculty of Medicine, University of Tokyo, Hongo, 113, Bunkyo-ku Tokyo
关键词
(Sea urchin embryo; Hemicentrotus pulcherrimus); DNA polymerase-γ; γ-Polymerase;
D O I
10.1016/0005-2787(79)90486-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A γ-like DNA polymerase devoid of DNA polymerase-α and -β activities was prepared from the nuclear fraction of blastulae of the sea urchin, Hemicentrotus pulcherrimus. The enzyme sedimented at the position of an approximate sedimentation coefficient of 3.3 S under high salt conditions by sucrose gradient centrifugation. An isoelectric point was determined to be pH 5.8. The enzyme activity was sensitive to sulfhydryl blocking reagents. Poly(rA) · oligo (dT)12-18 followed by poly(dA) · oligo(dT)12-18 was effectively utilized as a template-primer. From the above results, this polymerase seems to resemble the vertebrate DNA polymerase-γ. © 1979.
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页码:17 / 28
页数:12
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