PREPARATION OF DIPEPTIDYL AMINOPEPTIDASE-IV FOR POLYPEPTIDE SEQUENCING

被引：13

作者：

KRUTZSCH, HC

PISANO, JJ

机构：

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 576卷 / 02期

关键词：

Dipeptidyl aminopeptidase; Polypeptide sequence;

D O I：

10.1016/0005-2795(79)90403-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dipeptidyl aminopeptidase IV is a dipeptidylpeptide hydrolase (EC 3.4.14) which hydrolyzes bonds at the carboxyl group of proline releasing X-Pro dipeptides from the amino-terminus of polypeptides. The enzyme was purified 440-fold in 37% yield from swine kidney by ammonium sulfate fractionation, DEAE-cellulose chromatography, gel filtration and affinity chromatography with dipeptide-substituted Sepharose 4B. The enzyme released X-Pro from all X-Pro-β-naphthylamides and polypeptides tested. The released dipepeptides were not further degraded, and were readily identified in digests. The enzyme is suitable for use in the dipeptidyl aminopeptidase method for sequence analysis of polypeptides. © 1979.

引用

页码：280 / 289

页数：10

共 20 条

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