MEASUREMENT OF PENICILLIN ACYLASE AND CEPHALOSPORIN ACYLASE IN FERMENTATION BROTHS

被引：2

作者：

SPLIID, NH ^{[1
]}

EMBORG, C ^{[1
]}

机构：

[1] TECH UNIV DENMARK,DEPT APPL BIOCHEM,DK-2800 LYNGBY,DENMARK

来源：

BIOTECHNOLOGY LETTERS | 1979年 / 1卷 / 09期

关键词：

D O I：

10.1007/BF01386700

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The p-dimethylamino-benzaldehyde method of Bomstein and Evans, the ninhydrin method of Marrelli and the new p-dimethyl-amino-benzaldehyde method of Kornfeld were evaluated for analysis of penicillin-V acylase and cephalosporin-V acylase in fermentation broths. The ninhydrin method was the method of choice for cephalosporin-V acylase, whereas the Kornfeld method had certain advantages with respect to penicillin-V acylase. © 1979 Kluwer Academic Publishers.

引用

页码：359 / 364

页数：6

共 6 条

[1] AUTOMATED COLORIMETRIC DETERMINATION OF 6-AMINOPENICILLANIC ACID IN FERMENTATION MEDIA [J].

BOMSTEIN, J ;

EVANS, WG .

ANALYTICAL CHEMISTRY, 1965, 37 (04) :576-&

[2] ROLE OF PENICILLIN ACYLASE IN RESISTANCE OF GRAM-NEGATIVE BACTERIA TO PENICILLINS [J].

COLE, M ;

SUTHERLAND, R .

JOURNAL OF GENERAL MICROBIOLOGY, 1966, 42 (03) :345-+

[3]

HASH JH, 1975, METHODS ENZYMOLOGY, V43

[4] NEW COLORIMETRIC METHOD FOR DETERMINATION OF 6-AMINOPENICILLANIC ACID [J].

KORNFELD, JM .

ANALYTICAL BIOCHEMISTRY, 1978, 86 (01) :118-126

[5] COLORIMETRIC METHOD FOR DETERMINATION OF 7-AMINOCEPHALOSPORANIC ACID (7-ACA) AND RELATED COMPOUNDS [J].

MARRELLI, LP .

JOURNAL OF PHARMACEUTICAL SCIENCES, 1968, 57 (12) :2172-&

[6]

Vandamme E J, 1974, Adv Appl Microbiol, V17, P311, DOI 10.1016/S0065-2164(08)70563-X

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