DEACYLATION OF THE HEMAGGLUTININ OF INFLUENZA-A/AICHI/2/68 HAS NO EFFECT ON MEMBRANE-FUSION PROPERTIES

被引：62

作者：

STEINHAUER, DA

WHARTON, SA

WILEY, DC

SKEHEL, JJ

机构：

[1] NATL INST MED RES,MILL HILL,LONDON NW7 1AA,ENGLAND

[2] HARVARD UNIV,DEPT BIOCHEM & MOLEC BIOL,CAMBRIDGE,MA 02138

来源：

VIROLOGY | 1991年 / 184卷 / 01期

关键词：

D O I：

10.1016/0042-6822(91)90867-B

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The effects of deacylating the H3 influenza hemagglutinin (HA) on its membrane fusion activity were investigated. Chemical deacylation caused no change in the ability of HA to fuse liposomesin vitro. Site-specific mutagenesis of the three cysteine residues in the cytoplasmic tail singly, or in combination, showed that all three were palmitoylated. Substitution of one, two, or all three cysteines with serine and subsequent lack of palmitoylation at mutated sites had no effect on the pH of the conformational change in HA required for fusion activity or the extent of fusion activity. © 1991 Academic Press, Inc.

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页码：445 / 448

页数：4

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