THE TATA-BINDING PROTEIN PARTICIPATES IN TFIIIB ASSEMBLY ON TRANSFER-RNA GENES

被引：59

作者：

HUET, J ^{[1
]}

SENTENAC, A ^{[1
]}

机构：

[1] CENS,DBCM,SERV BIOCHIM & GENET MOLEC,F-91191 GIF SUR YVETTE,FRANCE

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 24期

关键词：

D O I：

10.1093/nar/20.24.6451

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The TATA-binding protein TBP has been recently recognized as a general class III transcription factor. Using the gel shift assay to monitor initiation complex assembly on a yeast tRNA gene, we show that TBP is required for the TFIIIC-dependent assembly of TFIIIB. TFIIIB depleted of TBP by a simple chromatographic step does not bind stably to the TFIIIC-tDNA complex. Addition of yeast or human recombinant TBP allows the formation of a TFIIIB-TBP-TFIIIC-tDNA complex. The presence of TBP in the complex was inferred from the effect of anti-TBP antibodies and from the different migration properties of TFIIIB-TBP-tDNA complexes formed with yeast or human TBP.

引用

页码：6451 / 6454

页数：4

共 28 条

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