EFFECT OF 2 CONSENSUS SEQUENCES PRECEDING THE TRANSLATION INITIATOR CODON ON GENE-EXPRESSION IN PLANT-PROTOPLASTS

被引：105

作者：

GUERINEAU, F ^{[1
]}

LUCY, A ^{[1
]}

MULLINEAUX, P ^{[1
]}

机构：

[1] JOHN INNES INST,JOHN INNES CTR PLANT SCI RES,NORWICH NR4 7UH,NORFOLK,ENGLAND

来源：

PLANT MOLECULAR BIOLOGY | 1992年 / 18卷 / 04期

关键词：

EXPRESSION CASSETTE; GENE EXPRESSION; PROTOPLASTS; TRANSLATION INITIATION;

D O I：

10.1007/BF00020027

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Expression cassettes containing a duplicated cauliflower mosaic virus (CaMV) 35S promoter fused to a polylinker preceded by the CCACCATGG and AACAATGG sequences were constructed. These two sequences correspond to the consensus sequences around the translation start condons in vertebrates and plants respectively. Translational fusions were made with the beta-glucuronidase-coding sequence and transient expression was recorded in tobacco mesophyll protoplasts. Approximately three times more GUS activity was found in protoplasts incubated with the constructs harbouring translational fusions as compared to a control harbouring a transcriptional fusion. No significant difference was observed between GUS activities obtained with the two consensus sequences.

引用

页码：815 / 818

页数：4

共 18 条

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