EQUINE PROTOZOAL MYELOENCEPHALITIS - ANTIGEN-ANALYSIS OF CULTURED SARCOCYSTIS-NEURONA MEROZOITES

被引：91

作者：

GRANSTROM, DE

DUBEY, JP

DAVIS, SW

FAYER, R

FOX, JC

POONACHA, KB

GILES, RC

COMER, PF

机构：

[1] Department of Veterinary Science, University of Kentucky, Lexington

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 1993年 / 5卷 / 01期

关键词：

D O I：

10.1177/104063879300500118

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Antigens of cultured Sarcocystis neurona merozoites were examined using immunoblot analysis. Blotted proteins were probed with S. cruzi, S. muris, and S. neurona antisera produced in rabbits, S. fayeri (pre- and post-infection) and S. neurona (pre- and post-inoculation) sera produced in horses, immune sera from 7 histologically confirmed cases of equine protozoal myeloencephalitis (EPM), and pre-suckle serum from a newborn foal. Eight proteins, 70, 24, 23.5, 22.5, 13, 11, 10.5, and 10 Kd, were detected only by S. neurona antiserum and/or immune serum from EPM-affected horses. Equine sera were titered by the indirect immunofluorescent antibody (IFA) method using air-dried, cultured S. neurona merozoites. Anti-Sarcocystis IFA titers were found in horses with or without EPM. Serum titers did not correspond to the number of specific bands recognized on immunoblots.

引用

页码：88 / 90

页数：3