SCREENING RETROVIRAL PACKAGING CELLS FOR HIGHLY EFFICIENT VIRUS PRODUCTION BY USING A COMBINED SELECTION PROCEDURE

被引：7

作者：

WIRTH, M ^{[1
]}

GRANNEMANN, R ^{[1
]}

KLEHR, D ^{[1
]}

HAUSER, H ^{[1
]}

机构：

[1] GESELL BIOTECHNOL FORSCH MBH,GENET EUKARYOTES,D-38124 BRAUNSCHWEIG,GERMANY

来源：

JOURNAL OF VIROLOGY | 1994年 / 68卷 / 01期

关键词：

D O I：

10.1128/JVI.68.1.566-569.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To facilitate the screening for clones of transfected packaging cells producing a high yield of recombinant retrovirus, we present a fast and simple method for the isolation of overexpressing cells. By this method the efficiency of virus production can generally be enhanced 10- to 100-fold by application of high selection pressure. Cell lines which exhibit titers of up to 10(8) CFU/ml mere obtained.

引用

页码：566 / 569

页数：4

共 20 条

[1] THE PROKARYOTIC NEOMYCIN-RESISTANCE-ENCODING GENE ACTS AS A TRANSCRIPTIONAL SILENCER IN EUKARYOTIC CELLS [J].

ARTELT, P ;

GRANNEMANN, R ;

STOCKING, C ;

FRIEL, J ;

BARTSCH, J ;

HAUSER, H .

GENE, 1991, 99 (02) :249-254

[2] DEVELOPMENT OF A HIGH-TITER RETROVIRUS PRODUCER CELL-LINE CAPABLE OF GENE-TRANSFER INTO RHESUS-MONKEY HEMATOPOIETIC STEM-CELLS [J].

BODINE, DM ;

MCDONAGH, KT ;

BRANDT, SJ ;

NEY, PA ;

AGRICOLA, B ;

BYRNE, E ;

NIENHUIS, AW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (10) :3738-3742

[3] SAFE AND EFFICIENT GENERATION OF RECOMBINANT RETROVIRUSES WITH AMPHOTROPIC AND ECOTROPIC HOST RANGES [J].

DANOS, O ;

MULLIGAN, RC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (17) :6460-6464

[4] NEW RETROVIRUS HELPER-CELLS WITH ALMOST NO NUCLEOTIDE-SEQUENCE HOMOLOGY TO RETROVIRUS VECTORS [J].

DOUGHERTY, JP ;

WISNIEWSKI, R ;

YANG, S ;

RHODE, BW ;

TEMIN, HM .

JOURNAL OF VIROLOGY, 1989, 63 (07) :3209-3212

[5] PING-PONG AMPLIFICATION OF A RETROVIRAL VECTOR ACHIEVES HIGH-LEVEL GENE-EXPRESSION - HUMAN GROWTH-HORMONE PRODUCTION [J].

KOZAK, SL ;

KABAT, D .

JOURNAL OF VIROLOGY, 1990, 64 (07) :3500-3508

[6] AUTOCRINE STIMULATION AFTER TRANSFER OF THE GRANULOCYTE MACROPHAGE COLONY-STIMULATING FACTOR GENE AND AUTONOMOUS GROWTH ARE DISTINCT BUT INTERDEPENDENT STEPS IN THE ONCOGENIC PATHWAY [J].

LAKER, C ;

STOCKING, C ;

BERGHOLZ, U ;

HESS, N ;

DELAMARTER, JF ;

OSTERTAG, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (23) :8458-8462

[7] PRODUCTION OF HIGH-TITER HELPER VIRUS-FREE RETROVIRAL VECTORS BY COCULTIVATION OF PACKAGING CELLS WITH DIFFERENT HOST RANGES [J].

LYNCH, CM ;

MILLER, AD .

JOURNAL OF VIROLOGY, 1991, 65 (07) :3887-3890

[8] CONSTRUCTION OF A RETROVIRUS PACKAGING MUTANT AND ITS USE TO PRODUCE HELPER-FREE DEFECTIVE RETROVIRUS [J].

MANN, R ;

MULLIGAN, RC ;

BALTIMORE, D .

CELL, 1983, 33 (01) :153-159

[9] A SAFE PACKAGING LINE FOR GENE-TRANSFER - SEPARATING VIRAL GENES ON 2 DIFFERENT PLASMIDS [J].

MARKOWITZ, D ;

GOFF, S ;

BANK, A .

JOURNAL OF VIROLOGY, 1988, 62 (04) :1120-1124

[10] GENERATION OF HELPER-FREE AMPHOTROPIC RETROVIRUSES THAT TRANSDUCE A DOMINANT-ACTING, METHOTREXATE-RESISTANT DIHYDROFOLATE-REDUCTASE GENE [J].

MILLER, AD ;

LAW, MF ;

VERMA, IM .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (03) :431-437

← 1 2 →