CONFORMATION AND FIBRILLOGENESIS OF ALZHEIMER A-BETA PEPTIDES WITH SELECTED SUBSTITUTION OF CHARGED RESIDUES

被引:150
作者
FRASER, PE
MCLACHLAN, DR
SUREWICZ, WK
MIZZEN, CA
SNOW, AD
NGUYEN, JT
KIRSCHNER, DA
机构
[1] NATL RES COUNCIL CANADA,STEACIE INST MOLEC SCI,OTTAWA K1A 0R6,ON,CANADA
[2] UNIV WASHINGTON,DEPT NEUROPATHOL,SEATTLE,WA 98195
[3] HARVARD UNIV,CHILDRENS HOSP,SCH MED,BOSTON,MA 02115
[4] HARVARD UNIV,SCH MED,DEPT NEUROL,BOSTON,MA 02115
关键词
ALZHEIMERS DISEASE; AMYLOID A-BETA; ELECTRON MICROSCOPY; X-RAY DIFFRACTION; INFRARED SPECTROSCOPY;
D O I
10.1006/jmbi.1994.1704
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A key pathological feature of Alzheimer's disease (AD) is the formation and accumulation of amyloid fibers within the neurophil as senile plaques and in the walls of cerebral and meningeal blood vessels. The major component is the 39 to 42 residue amyloid beta protein (A beta), which an internal proteolytic fragment of the membrane-associated amyloid precursor protein. Aggregation of A beta into amyloid fibers that could be cytotoxic may be a factor in the AD-related neuronal loss. To understand the steps and molecular interactions involved in the transition from a soluble to fibrous form of A beta, and to test molecular models that postulate ion pairing between beta-strands, we have synthetized four peptides haring substitutions in specific, charged residues. These included an A beta fragment, residues 11 to 25, and having histidine-to-aspartate replacements at positions 13 (H13D) and 14 (H14D), an aspartate-to-lysine at position 23 (D23K) and a 28-mer full-length extracellular domain where the positive charge cluster at His13-His14-Gln15-Lys16 was replaced by an uncharged Gly13-Gly14-Gln15-Gly16 (GGQG). Fourier-transform infrared spectroscopy and fiber X-ray diffraction determined that the H13D and H14D substitutions had negligible effect on beta-sheet formation, suggesting that these residues are not critical for the intramolecular interactions necessary for folding in the beta-conformation. However, negative-stain electron microscopy revealed that the loss of the His13 or His14 resulted in only protofilament formation, suggesting that these residues are involved in amyloid fibril assembly By contrast, the D23K substitution virtually eliminated folding into a beta-sheet conformation, with appreciable secondary structure being detected only following extended incubation times. The complete absence of the centrally charged region GGQG arrested amyloid assembly at the protofilament stage and also reduced the stability of the beta-conformation, suggesting a contribution of Lys16 in maintaining secondary structure. While it has been conclusively demonstrated by previous investigations that amyloid formation is dependent to it large extent on hydrophobically driven interactions, our results indicate that charge-charge interactions function in concert with non-ionic interactions to stabilize the beta-sheet conformation and assembly of AD amyloid fibers.
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页码:64 / 73
页数:10
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