DETERMINATION OF THE ENANTIOMERS OF BUNOLOL IN HUMAN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON A CHIRAL AGP STATIONARY-PHASE AND IDENTIFICATION OF THEIR METABOLITES BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：19

作者：

LI, F ^{[1
]}

COOPER, SF ^{[1
]}

COTE, M ^{[1
]}

AYOTTE, C ^{[1
]}

机构：

[1] UNIV QUEBEC,INST NATL RECH SCI SANTE,POINTE CLAIRE H9R 1G6,PQ,CANADA

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 660卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00295-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic method using a chiral AGP column was developed to screen and determine the enantiomers of bunolol in human urine. The recovery of (+)- and (-)-bunolol from urine was 91.79-95.23% at different concentrations. The coefficients of variation (C.V.) were less than 2.1 and 2.3% for intra- and inter-assays, respectively. Urinary metabolites were detected using GC-MS after derivatization with N-methyl(trimethylsilyI)trifluroacetamide. The influences of pH and modifier on a chiral AGP column were studied.

引用

页码：327 / 339

页数：13

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