MEMBRANE DEFECTS OF THE TOLERANT-B CELL .1. FAILURE OF ANTIGEN-INDUCED CAPPING

被引:19
作者
ASHMAN, RF
NAOR, D
机构
[1] UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,DIV RHEUMATOL,LOS ANGELES,CA 90024
[2] HEBREW UNIV JERUSALEM,LAUTENBERG CTR GEN & TUMOR IMMUNOL,HADASSAH MED SCH,JERUSALEM 91000,ISRAEL
基金
美国国家卫生研究院;
关键词
D O I
10.1016/0008-8749(79)90008-X
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In order to study the membrane function of tolerant B antigen-binding cells, tolerance to the trinitrophenyl (TNP) determinant was induced in mice by injecting the reactive form of the hapten, trinitrobenzene sulfonic acid (TNBS). By appropriate transfer experiments, Fidler and Golub (J. Immunol. 112, 1891, 1974) had previously shown that this form of tolerance is a B-cell property, induced and expressed in the absence of T cells. Hapten inhibition demonstrated the TNP-specificity of receptors on TNP-donkey erythrocyte(TNP-D)-binding cells in tolerant and nontolerant mice. About 88% of these cells were B cells by immunofluorescence, and the remainder were T cells. In the tolerant mice, challenge with TNP-sheep erythrocytes failed to expand the TNP-binding population, but sheep erythrocyte binders and anti-sheep plaque-forming cells expanded normally. Despite little or no change in TNP-binding cell numbers after tolerance induction, the TNP-binding cells of tolerant animals could not cap their receptors, in contrast to the sheep erythrocyte-binding cells from the same animals which capped normally. Although there is no anti-TNP plaque-forming cell response when tolerogen and immunogen are given simultaneously, capping failure is not evident until 2-4 days after tolerogen exposure. By Day 7, substantial recovery of immune responsiveness had occurred, yet even 12 months after a single dose of tolerogen there was no restoration of capping. Thus despite the association of both capping failure and unresponsiveness with tolerogen exposure, these lymphocyte functional defects appeared not to be causally related. © 1979.
引用
收藏
页码:314 / 328
页数:15
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