THE T-ALPHA-2 NUCLEAR-PROTEIN BINDING-SITE FROM THE HUMAN T-CELL RECEPTOR ALPHA-ENHANCER FUNCTIONS AS BOTH A T-CELL SPECIFIC TRANSCRIPTIONAL ACTIVATOR AND REPRESSOR

T cell-specific expression of the human T cell receptor α (TCR-α) gene is regulated by the interaction of variable region promoter elements with a transcriptional enhancer that is located 4.5 kb 3’ of the TCR-α constant region (Caα) gene segment. The minimal TCR-α enhancer is composed of two nuclear protein binding sites, Tαl and Tα2, that are both required for the T cell-specific activity of the enhancer. The Tα1 binding site contains a consensus cAMP response element (CRE), and binds a set of ubiquitous nuclear proteins. The Tα2 binding site does not contain known transcriptional enhancer motifs. However, it binds at least two nuclear protein complexes, one of which is T cell specific. We now report that although the Tα2 nuclear protein binding site displays transcriptional activator activity in the context of the TCR-α enhancer, this site alone can function as a potent, T cell-specific transcriptional repressor when positioned either upstream, or downstream of several heterologous promoter and enhancer elements. These results demonstrate that a single nuclear protein binding site can function as a T cell-specific transcriptional activator or repressor element, depending upon the context in which it is located. © 1990, Rockefeller University Press., All rights reserved.