ISOLATION AND CHARACTERIZATION OF GRASS CARP (CTENOPHARYNGODON-IDELLUS) LIVER ALCOHOL-DEHYDROGENASE

被引：5

作者：

FONG, WP

机构：

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1991年 / 98卷 / 2-3期

关键词：

D O I：

10.1016/0305-0491(91)90182-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. Alcohol dehydrogenase was isolated from the liver of grass carp (Ctenopharyngodon idellus) by DEAE-cellulose and Affi-gel Blue agarose chromatography. 2. SDS-polyacrylamide gel electrophoresis of the purified enzyme showed a single protein band with subunit molecular weight of approx. 28,000. 3. Starch gel electrophoresis at pH 8.2 revealed at least three anodic bands of alcohol oxidizing activity. 4. The enzyme oxidized a number of primary and secondary alcohols and was inhibited by 4-methyl-pyrazole and o-phenanthroline. 5. The optimal pH for the oxidation of ethanol was around 10.5.

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页码：297 / 302

页数：6

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