CONTINUOUS-FLOW SENSOR STRATEGY COMPRISING A ROTATING BIOREACTOR AND A STATIONARY RING AMPEROMETRIC DETECTOR

被引：25

作者：

MATSUMOTO, K

BAEZA, JJB

MOTTOLA, HA

机构：

[1] OKLAHOMA STATE UNIV,DEPT CHEM,STILLWATER,OK 74078

[2] KYUSHU UNIV,FAC AGR,DEPT FOOD SCI & TECHNOL,FUKUOKA 812,JAPAN

[3] UNIV VALENCIA,FAC MED,DEPT ANALYT CHEM,VALENCIA,SPAIN

来源：

ANALYTICAL CHEMISTRY | 1993年 / 65卷 / 05期

关键词：

D O I：

10.1021/ac00053a025

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rotating immobilized-enzyme reactor and a static Pt-ring electrode have been designed In a sealed cell for use under continuous-flow/stopped-flow operation. The operating characteristics of the integrated bioreactor/detector unit have been studied for determination of glucose using immobilized glucose oxidase [EC 1. 1.3.41 and amperometric monitoring of the H2O2 produced. Determinations are based on the rate of response under stopped-flow conditions. The trend in values of the apparent Michaelis-Menten constant confirms the validity of the approach to sensitive determinations utilizing very small amounts of biocatalysts.

引用

页码：636 / 639

页数：4

共 11 条

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