COMPARATIVE-STUDY OF A BLOCKING ENZYME-LINKED-IMMUNOSORBENT-ASSAY AND THE IMMUNOPEROXIDASE MONOLAYER ASSAY FOR THE DETECTION OF ANTIBODIES TO THE PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS IN PIGS

被引：39

作者：

HOUBEN, S ^{[1
]}

CALLEBAUT, P ^{[1
]}

PENSAERT, MB ^{[1
]}

机构：

[1] STATE UNIV GHENT,FAC VET MED,VIROL LAB,B-9820 MERELBEKE,BELGIUM

来源：

JOURNAL OF VIROLOGICAL METHODS | 1995年 / 51卷 / 01期

关键词：

BLOCKING ELISA; IMMUNOPEROXIDASE MONOLAYER ASSAY; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS; (PIG);

D O I：

10.1016/0166-0934(94)00135-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A blocking enzyme-linked immunosorbent assay (ELISA) for detecting antibodies to the porcine reproductive and respiratory syndrome virus (PRRSV) in pig sera was developed and compared with the immunoperoxidase monolayer assay (IPMA), the most widely used serological diagnostic test in Europe. The blocking ELISA was specific and more sensitive than the IPMA when applied to field sera and to sera which were collected early after an experimental infection with PRRSV. Problems with high background activity as observed in IPMA or indirect ELISA were not encountered.

引用

页码：125 / 128

页数：4

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