LOCALIZATION OF FUNCTIONAL DOMAINS IN THE ESCHERICHIA-COLI COPROGEN RECEPTOR FHUE AND THE PSEUDOMONAS-PUTIDA FERRIC-PSEUDOBACTIN-358 RECEPTOR PUPA

被引:7
作者
BITTER, W
VANLEEUWEN, IS
DEBOER, J
ZOMER, HWM
KOSTER, MC
WEISBEEK, PJ
TOMMASSEN, J
机构
[1] UNIV UTRECHT,DEPT MOLEC CELL BIOL,3584 CH UTRECHT,NETHERLANDS
[2] UNIV UTRECHT,INST BIOMEMBRANES,3584 CH UTRECHT,NETHERLANDS
来源
MOLECULAR & GENERAL GENETICS | 1994年 / 245卷 / 06期
关键词
PSEUNOMONAS; SIDEROPHORE; OUTER MEMBRANE; RECEPTOR; TONB;
D O I
10.1007/BF00297276
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transport of ferric-siderophores across the outer membrane of gram-negative bacteria is mediated by specific outer membrane receptors. To localize the substrate-binding domain of the ferric-pseudobactin 358 receptor, PupA, of Pseudomonas putida WCS358, we constructed chimeric receptors in which different domains of PupA were replaced by the corresponding domains of the related ferric-pseudobactin receptors PupB and PupX, or the coprogen receptor FhuE of Escherichia coli. None of the chimeric proteins composed of pseudobactin receptor domains facilitated growth on any of the original substrates, or they showed only an extremely low efficiency. However, these receptors enabled cells of Pseudomonas BN8 to grow on media supplemented with uncharacterized siderophore preparations. These siderophore preparations were isolated from the culture supernatant of WCS358 cells carrying plasmids that contain genes of Pseudomonas B10 required for the biosynthesis of pseudobactin B10. Hybrid proteins that contained at least the amino-terminal 516 amino acids of mature FhuE were active as a receptor for coprogen and interacted with the E. coli TonB protein. A chimeric PupA-FhuE protein, containing the amino-terminal 94 amino acids of mature PupA, was also active as a coprogen receptor, but only in the presence of Pseudomonas TonB. It is concluded that the carboxy-terminal domain of ferric-pseudobactin receptors is important, but not sufficient, for ligand interaction, whereas binding of coprogen by the FhuE receptor is not dependent on this domain. Apparently, the ligand-binding sites of different receptors are located in different regions of the proteins. Furthermore, species-specific TonB binding by the PupA receptor is dependent on the amino-terminal domain of the receptor.
引用
收藏
页码:694 / 703
页数:10
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