GAS-SENSING INTERNAL ENZYME FIBER OPTIC BIOSENSOR FOR HYDROGEN-PEROXIDE

Feasibility is demonstrated for a novel gas-sensing, internal enzyme biosensing scheme for the selective measurement of hydrogen peroxide. Two horseradish peroxidase catalyzed reactions are evaluated for the detection of hydrogen peroxide as it crosses a microporous Teflon membrane at 37-degrees-C. The rate al which hydrogen peroxide crosses the membrane is determined by either a fluorescence or chemiluminescence measurement and this rate is related to the concentration of hydrogen peroxide in the sample solution. Detection limits of 0.7 mM and 10 muM are estimated for the fluorescence and chemiluminescence methods, respectively. Selectivity is demonstrated for hydrogen peroxide over ascorbic acid, uric acid and tyrosine.