METABOTROPIC GLUTAMATE-RECEPTOR AGONIST DCG-IV AS NMDA RECEPTOR AGONIST IN IMMATURE RAT HIPPOCAMPAL-NEURONS

被引：67

作者：

WILSCH, VW ^{[1
]}

PIDOPLICHKO, VI ^{[1
]}

OPITZ, T ^{[1
]}

SHINOZAKI, H ^{[1
]}

REYMANN, KG ^{[1
]}

机构：

[1] TOKYO METROPOLITAN INST MED SCI,DEPT PHARMACOL,BUNKYO KU,TOKYO 113,JAPAN

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY | 1994年 / 262卷 / 03期

关键词：

HIPPOCAMPAL NEURON; ISOLATED; CONCENTRATION JUMP; GLUTAMATE RECEPTOR; NMDA RECEPTOR; CHEMICALLY GATED CURRENT;

D O I：

10.1016/0014-2999(94)90743-9

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The electrophysiological action of (2S,1'R;2'R,3'R)-2-(2,3-dicarboxycyclopropyl)glycine (DCG-IV) on the NMDA receptor was investigated with the help of concentration-jump experiments on freshly dissociated hippocampal CA1 and CA3 neurons of rats. Inward currents elicited by a pulse of DCG-IV were insensitive to 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and could be blocked by D-(-)-2-amino-5-phosphonopentanoic acid (D-AP5) and magnesium and enhanced by glycine. The substance displayed cross-desensitization with NMDA but not with kainate or(RS)-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA). Dose-response curves showed that DCG-IV was weaker than NMDA but more potent than glutamate in eliciting agonist-gated currents. From these data we conclude that DCG-IV has to be used with caution as a tool for the investigation of metabotropic glutamate receptors.

引用

页码：287 / 291

页数：5

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