THE ANTITUMOR ACTION OF SEMINAL RIBONUCLEASE AND ITS QUATERNARY CONFORMATIONS

被引：69

作者：

CAFARO, V ^{[1
]}

DELORENZO, C ^{[1
]}

PICCOLI, R ^{[1
]}

BRACALE, A ^{[1
]}

MASTRONICOLA, MR ^{[1
]}

DIDONATO, A ^{[1
]}

DALESSIO, G ^{[1
]}

机构：

[1] UNIV NAPLES FEDERICO II,DIPARTIMENTO CHIM ORGAN & BIOL,I-80134 NAPLES,ITALY

来源：

FEBS LETTERS | 1995年 / 359卷 / 01期

关键词：

RIBONUCLEASE; ANTITUMOR; PROTEIN CONFORMATION; PROTEIN ENGINEERING;

D O I：

10.1016/0014-5793(94)01450-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It has been previously shown that the antitumor action of bovine seminal ribonuclease (BS-RNase) is dependent on its dimeric structure. However, two distinct quaternary structures, each in equilibrium with the other, have been described for the enzyme: one in which the two subunits exchange their N-terminal ends, the other with no exchange. Antitumor activity assays, carried out on homogeneous quaternary forms of the enzyme, as well as on dimeric mutants of bovine pancreatic RNase A, reveal that another structural determinant of the antitumor activity of BS-RNase is the exchange of N-terminal ends between subunits.

引用

页码：31 / 34

页数：4

共 15 条

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