CONSTRUCTION AND ANALYSIS OF INFECTIOUS TRANSCRIPTS SYNTHESIZED FROM FULL-LENGTH CDNA CLONES OF BOTH GENOMIC RNAS OF PEA EARLY BROWNING VIRUS

被引：22

作者：

MACFARLANE, SA ^{[1
]}

WALLIS, CV ^{[1
]}

TAYLOR, SC ^{[1
]}

GOULDEN, MG ^{[1
]}

WOOD, KR ^{[1
]}

DAVIES, JW ^{[1
]}

机构：

[1] UNIV BIRMINGHAM,DEPT MICROBIOL,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND

来源：

VIROLOGY | 1991年 / 182卷 / 01期

关键词：

D O I：

10.1016/0042-6822(91)90655-U

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Full-length cDNA clones of both RNAs of pea early browning virus have been constructed. Synthetic transcripts derived in vitro from these clones are infectious when inoculated onto plants. Electron microscopy revealed the presence of virions in transcript-inoculated plants, and both purified RNA and virions isolated from such plants could be used to infect other plants. Transcripts of RNA1 alone were able to replicate and spread systemically which is a characteristic of members of the tobravirus group of plant viruses. © 1991.

引用

页码：124 / 129

页数：6

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