ESTABLISHMENT AND CHARACTERIZATION OF C-TYPE RNA VIRUS-PRODUCING CELL-LINES FROM RADIATION-INDUCED MURINE OSTEOSARCOMAS

被引:11
作者
ERFLE, V
SCHULTEOVERBERG, S
MARQUART, KH
ADLER, ID
LUZ, A
机构
[1] Institut für Biologie, Gesellschaft für Strahlen- und Umweltforschung mbH München, Neuherberg
关键词
C-type RNA virus; Cell line; Mice; Radiation-induced osteosarcoma;
D O I
10.1007/BF00422495
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Eight cell lines were established from murine osteosarcomas induced in vivo with the radionuclides 224Ra and 227Th. They have been compared by light and electron microscopy, by karyology, and by their growth properties. The morphology, the growth pattern, and the ability to induce tumors in mice indicate that five of them are tumor cell lines. Chromosome studies demonstrated that the five cell lines have marker chromosomes. The other cell lines only showed some criteria generally used to score for transformation of fibroblasts and they may be derived from stromal cells. All cell lines release virus particles in the culture fluid which have the typical properties of RNA tumor viruses. They possess C-type morphology, a density of 1.16-1.18 g/cm3, a 60-70 S RNA, a RNA dependent DNA polymerase and they induce syncytia in rat XC cells. The possible significance of these virus particles in radiation osteosarcomagenesis is discussed. © 1979 Springer-Verlag.
引用
收藏
页码:149 / 162
页数:14
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