THE CD-CHELEX ASSAY - A NEW SENSITIVE METHOD TO DETERMINE METALLOTHIONEIN CONTAINING ZINC AND CADMIUM

被引：89

作者：

BARTSCH, R ^{[1
]}

KLEIN, D ^{[1
]}

SUMMER, KH ^{[1
]}

机构：

[1] INST TOXIKOL GESELL STRAHLEN & UMWELTFORSCH,INGOLSTADTER LANDSTR 1,W-8042 NEUHERBERG,GERMANY

来源：

ARCHIVES OF TOXICOLOGY | 1990年 / 64卷 / 03期

关键词：

Cadmium; Cd-saturation assay; Chelex; Metallothionein; Zinc;

D O I：

10.1007/BF02010722

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

A rapid and sensitive one-vial procedure to determine metallothionein (MT) containing zinc (Zn) and cadmium (Cd) is described. New features of this Cd-saturation method are: high molecular weight Cd-binding proteins are denatured by treatment with acetonitrile (50% final concentration), and excess of Cd is bound to a cation exchange resin (Chelex-100). With this method, MT has been measured, e.g. in liver of control and zinc- or cadmium-treated rats, in human liver and in cultured human fibroblasts down to absolute amounts of 0.1 μg. The Cd-Chelex assay is 10 times more sensitive than the established Cd-heme assay (Dieter et al. 1986) and therefore is particularly suitable to quantify MT in small tissue samples (e.g., liver biopsies of a few milligrams) and in extrahepatic tissues or cell cultures with low MT concentrations. © 1990 Springer-Verlag.

引用

页码：177 / 180

页数：4

共 12 条

[1] METAL-BINDING IN METALLOTHIONEINS - COMPETITION FOR CADMIUM AND ZINC BETWEEN CHELEX-100 AND METAL-BINDING SITES IN METALLOTHIONEIN [J].

CAI, W ;

STILLMAN, MJ .

INORGANICA CHIMICA ACTA-BIOINORGANIC CHEMISTRY, 1988, 152 (02) :111-115

[2] DETERMINATION OF CD-THIONEIN IN BIOLOGICAL-MATERIALS - COMPARATIVE STANDARD RECOVERY BY 5 CURRENT METHODS USING PROTEIN NITROGEN FOR STANDARD CALIBRATION [J].

DIETER, HH ;

MULLER, L ;

ABEL, J ;

SUMMER, KH .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 1986, 85 (03) :380-388

[3] EVALUATION OF THE CD HEMOGLOBIN AFFINITY ASSAY FOR THE RAPID-DETERMINATION OF METALLOTHIONEIN IN BIOLOGICAL TISSUES [J].

EATON, DL ;

TOAL, BF .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 1982, 66 (01) :134-142

[4]

FOULKES EC, 1982, BIOL ROLES METALLOTH

[5] METALLOTHIONEIN CONTENT AND ZINC STATUS IN VARIOUS TISSUES OF RATS TREATED WITH IODOACETIC ACID AND ZINC [J].

HEILMAIER, HE ;

SUMMER, KH .

ARCHIVES OF TOXICOLOGY, 1985, 56 (04) :247-251

[6]

Kagi J.H.R., 1987, METALLOTHIONEIN, VII

[7] CHARACTERIZATION OF ISOPROTEIN PATTERNS IN TISSUE-EXTRACTS AND ISOLATED SAMPLES OF METALLOTHIONEINS BY REVERSE-PHASE HIGH-PRESSURE LIQUID-CHROMATOGRAPHY [J].

KLAUSER, S ;

KAGI, JHR ;

WILSON, KJ .

BIOCHEMICAL JOURNAL, 1983, 209 (01) :71-80

[8]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[9] INDUCTION OF METALLOTHIONEIN IN RAT-TISSUES FOLLOWING SUBCHRONIC EXPOSURE TO MERCURY SHOWN BY RADIOIMMUNOASSAY [J].

NOLAN, CV ;

SHAIKH, ZA .

BIOLOGICAL TRACE ELEMENT RESEARCH, 1987, 12 :419-428

[10] DETERMINATION OF METALLOTHIONEIN IN TISSUES BY RADIOIMMUNOASSAY AND BY CADMIUM SATURATION METHOD [J].

NOLAN, CV ;

SHAIKH, ZA .

ANALYTICAL BIOCHEMISTRY, 1986, 154 (01) :213-223

← 1 2 →