ELUCIDATION OF CDNA SEQUENCES FOR METALLOTHIONEINS FROM RAINBOW-TROUT, STONE LOACH AND PIKE LIVER USING THE POLYMERASE CHAIN-REACTION

被引：53

作者：

KILLE, P

STEPHENS, PE

KAY, J

机构：

[1] UNIV WALES COLL CARDIFF,COLL CARDIFF,DEPT BIOCHEM,CARDIFF CF1 1ST,WALES

[2] CELLTECH LTD,SLOUGH,ENGLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1089卷 / 03期

关键词：

FISH METALLOTHIONEIN; CDNA; POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; 3' UNTRANSLATED VARIATION;

D O I：

10.1016/0167-4781(91)90187-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Metallothionein cDNAs were generated from the livers of three fish species and amplified by PCR. Two distinct coding sequences (A and B) were elucidated for rainbow trout metallothioneins but single isoforms were encoded by genes isolated from the stone loach and pike. Different sized transcripts were observed both with stone loach and pike but these were accounted for by altered lengths of 3' untranslated regions.

引用

页码：407 / 410

页数：4

共 16 条

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