EXPRESSION OF THE FUNGAL CYTOCHROME-P-450NOR CDNA IN ESCHERICHIA-COLI

被引：11

作者：

OBIKA, K

TOMURA, D

FUKAMIZU, A

SHOUN, H

机构：

[1] Univ Tsukuba, Dept Appl Biochem, Tsukuba, Ibaraki

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 196卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.2387

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We succeeded in expressing the unique cytochrome P-450 (P-450), nitric oxide reductase (P-450nor), in Escherichia coli by utilizing P-450nor cDNA and the expression vector pKK233-2 or pUC19. The expression was confirmed by Western blot analysis and by detecting the unique nitric oxide reductase activity. The expressed protein was recovered in the soluble fraction of transformed cells, supporting that P450nor is the first soluble P-450 of eukaryote. The results also provided conclusive evidence that the enzymatic reaction depends solely on P450nor without support of any other electron transferring components. © 1993 Academic Press. All rights reserved.

引用

页码：1255 / 1260

页数：6

共 14 条

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