TEMPORARY CULTURE IN ISOLEUCINE-FREE MEDIUM ENHANCES TRANSFORMATION OF 10T1/2 CELLS BY N-METHYL-N'-NITRO-N-NITROSOGUANIDINE (MNNG)

被引：12

作者：

GRISHAM, JW

GREENBERG, DS

SMITH, GJ

KAUFMAN, DG

机构：

[1] Department of Pathology University, North Carolina School of Medicine Chapel Hill

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 87卷 / 04期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0006-291X(79)80001-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Arrest of proliferation of C3H 10T1/2 cells in isoleucine-free medium sensitizes them to the transforming action of MNNG when they are exposed to this chemical after release. Exposure of 10T1/2 cells to MNNG for 30 min causes three to four times the rate of transformation in these cells when they are recycling following arrest of proliferation by isoleucine deficiency than does similar treatment of the same cells when they are recycling after arrest of proliferation by growth to confluence. Rates of transformation differ even though the magnitude of binding of MNNG to DNA is similar in cells recycling after suppression of proliferation by either method. © 1979 Academic Press, Inc. All rights of reproduction in any form reserved.

引用

页码：969 / 975

页数：7

共 9 条

[1]

BERTRAM JS, 1974, CANCER RES, V34, P526

[2] CAFFEINE INHIBITS EXCISION OF 7-BROMOMETHYLBENZ (A) ANTHRACENE-DNA ADDUCTS FROM EXPONENTIALLY GROWING BUT NOT FROM STATIONARY PHASE CHINESE-HAMSTER CELLS [J].