PLASMA NEUROTENSIN IN THE CONSCIOUS PIG - RELEASE BY INDIVIDUAL FOOD COMPONENTS AND EFFECTS ON EXOCRINE PANCREAS SECRETION

被引：19

作者：

CUBER, JC

PHILIPPE, C

ABELLO, J

CORRING, T

LEVENEZ, F

CHAYVIALLE, JA

机构：

[1] INSERM U45, Hopital E. Herriot, Lyon

来源：

PANCREAS | 1990年 / 5卷 / 03期

关键词：

Exocrine pancreas secretion; Neurotensin release; Pig;

D O I：

10.1097/00006676-199005000-00010

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

In conscious pigs, i.v. infusion of serial doses of neurotensin (NT, actual doses of 0.24-59.4 pmol kg-1min-1) on a background of secretin resulted in a linear increase of plasma NT-like immunoreactivity (NT-LI), as measured with an antiserum that requires the biologically active C-terminal part of the molecule for recognition. The NT infusions were accompanied by a dose-related increase of pancreatic volume and bicarbonate and protein output. The threshold plasma NT-LI concentrations for significant increases of pancreatic enzymes and of pancreatic fluid and bicarbonate were 46.6 ± 7.0 and 161.3 ± 10.8 pM, respectively. Food intake was followed by a sharp pancreatic response and a progressive increase of plasma NT-LI level to a peak of about 27.7 ± 3.0 pM from the basal level 11.8 ± 1.6 pM. The carbohydrate fraction of the meal was predominantly responsible for the NT release observed after meal intake. High-performance liquid chromatography analysis of plasma samples collected during NT infusion or after meal ingestion revealed one immunoreactive peak coeluting with intact NT. Pancreatic polypeptide was released on infusion of high, supraphysiological doses of NT, while plasma somatostatin remained at low basal values. It is concluded that in the pig, intact NT is released after a standard meal predominantly through the carbohydrate fraction of the diet, NT is a stimulant of exocrine pancreas secretion, and the modest pancreatic response to physiologic increments of plasma NT-LI on peptide infusion is not attributable to indirect inhibition of the exocrine pancreas by NT-released pancreatic polypeptide or somatostatin. © 1990 Raven Press, New York.

引用

页码：306 / 313

页数：8

共 31 条

[1]

Carraway R., Leeman S.E., Characterization of radioimmu-noassayable neurotensin in the rat, J Biol Chem, 251, pp. 7045-7052, (1976)

[2]

Hammer R.A., Leeman S.E., Carraway R., Williams R.H., Iso-lation of human intestinal neurotensin, J Biol Chem, 255, pp. 2476-2480, (1980)

[3]

Helmstaedter V., Taugner C., Feurle G.E., Forssmann W.G., Localization of neurotensin-immunoreactive cells in the small intestine of man and various mammals, Histochemis-Try, 53, pp. 35-41, (1977)

[4]

Mashford M.L., Nilsson G., Rokaeus A., Rosell S., The effect of food ingestion on circulating neurotensin-like immunoreactivity (NTLI) in the human, Acta Physiol Scand, 104, pp. 244-246, (1978)

[5]

Konturek S.J., Jaworek J., Cieszkowski M., Pawlik W., Kania J., Bloom S.R., Comparison of effects of neurotensin and fat on pancreatic stimulation in dogs, Am J Physiol, 244, pp. G590-G598, (1983)

[6]

Shaw C., Buchanan K.D., Intact neurotensin (NT) in human plasma: Response to oral feeding, Regul Pept, 7, pp. 145-153, (1983)

[7]

Rosell S., Rokaeus A., The effect of ingestion of amino acids, glucose and fat on circulating neurotensin-like immunoreactivity (NTLI) in man, Acta Physiol Scand, 107, pp. 263-267, (1979)

[8]

Ferris C.F., Hammer R.A., Leeman S.E., Elevation of plasma neurotensin during lipid perfusion of rat small intestine, Peptides, 2, pp. 263-266, (1981)

[9]

Yamashita Y., Hoist Pedersen J., Stadil F., Extraction of neurotensin-like immunoreactivities from porcine ileal mucosa, Peptides, 8, pp. 639-643, (1987)

[10]

Feurle G.E., Hofmann G., Carraway R., Baca I., Reproduction of postprandial neurotensin plasma levels by intravenous neurotensin and the effect of neurotensin on exocrine pancreatic secretion in humans, Pancreas, 1, pp. 329-334, (1986)

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