DETERMINATION OF CEFATRIZINE IN SERUM AND URINE BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：19

作者：

CROMBEZ, E

VANDERWEKEN, G

VANDENBOSSCHE, W

DEMOERLOOSE, P

机构：

[1] Department of Pharmaceutical Chemistry and Drug Quality Control, State University of Gent, Faculty of Pharmaceutical Sciences, B-9000 Ghent

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 173卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(01)80456-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A fas and simple high-performance liquid chromatographic procedure for the determination of cefatrizine, an orally active cephalosporin, in serum and urine is proposed. Reversed-phase liquid chromatography on an octadecylsilane chemically bonded microparticulate packing, using methanol in 0.03 M sodium phosphate buffer (pH 5) as eluent, was used to separate and quantitate the antibiotic. The samples were analysed after deproteinization with trichloroacetic acid and injection of the clear supernatant. The accuracy and reproducibility of the procedure were investigated by determination of the cefatrizine content in spiked serum and urine samples, using cepharadine as the internal standard. © 1979.

引用

页码：165 / 173

页数：9

共 18 条

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