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MOLECULAR-CLONING AND EXPRESSION OF RABBIT PANCREATIC CHOLESTEROL ESTERASE

被引：26
作者：
COLWELL, NS
ALEMANGOMEZ, JA
KUMAR, BV
机构：
[1] VET ADM MED CTR,CTR GERIATR RES EDUC & CLIN,ST LOUIS,MO 63125
[2] ST LOUIS UNIV,DIV GERIATR MED,ST LOUIS,MO 63125
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1172卷 / 1-2期
关键词：
CHOLESTEROL ESTERASE; CDNA; EXPRESSION; PEST; (RABBIT PANCREAS);
D O I：
10.1016/0167-4781(93)90288-O
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Rabbit pancreatic cholesterol esterase (CEase, carboxyl ester lipase, EC 3.1.1.3) has been cloned from a lambdagt11 library of adult rabbit pancreatic cDNA. The open reading frame consists of 1788 nucleotides which encodes 576 amino acids of the functional protein and a 20 amino acid leader peptide. When compared to other species, the greatest homology is observed between residues 82-248 with little or no homology at the C-terminal end where proline-glutamate-serine-threonine (PEST) segments are a characteristic feature of the human CEase. Rabbit CEase (RCEase) retains the active-site serine (gxsxg), the active-site histidine and the tentative heparin binding site (KKRCLQ) at similar positions in comparison to pancreatic CEases of other species. When rabbit CEase cDNA is expressed in monkey kidney (COS-7) cells, enzymatic hydrolytic activity is detected in the growth medium as is a 67 kDa protein by Western blotting with polyclonal anti-CEase antibody. Northern blot analysis shows two mRNA (2.2 and 3.2 kb) species.
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页码：175 / 180
页数：6
相关论文
共 40 条
[1]   STEROL BALANCE AND CHOLESTEROL ABSORPTION IN INBRED STRAINS OF RABBITS HYPORESPONSIVE OR HYPERRESPONSIVE TO DIETARY-CHOLESTEROL [J].
BEYNEN, AC ;
MEIJER, GW ;
LEMMENS, AG ;
GLATZ, JFC ;
VERSLUIS, A ;
KATAN, MB ;
VANZUTPHEN, LFM .
ATHEROSCLEROSIS, 1989, 77 (2-3) :151-157
[2]   BILE SALT-STIMULATED LIPASE IN HUMAN-MILK - EVIDENCE FOR ITS SYNTHESIS IN THE LACTATING MAMMARY-GLAND [J].
BLACKBERG, L ;
ANGQUIST, KA ;
HERNELL, O .
FEBS LETTERS, 1987, 217 (01) :37-41
[3]   RECEPTOR-LIKE FUNCTION OF HEPARIN IN THE BINDING AND UPTAKE OF NEUTRAL LIPIDS [J].
BOSNER, MS ;
GULICK, T ;
RILEY, DJS ;
SPILBURG, CA ;
LANGE, LG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (20) :7438-7442
[4]   THE MOLECULAR EVOLUTION OF GENES AND PROTEINS - A TALE OF 2 SERINES [J].
BRENNER, S .
NATURE, 1988, 334 (6182) :528-530
[5]   MOLECULAR MODELING OF PROTEIN-GLYCOSAMINOGLYCAN INTERACTIONS [J].
CARDIN, AD ;
WEINTRAUB, HJR .
ARTERIOSCLEROSIS, 1989, 9 (01) :21-32
[6]   ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].
CHIRGWIN, JM ;
PRZYBYLA, AE ;
MACDONALD, RJ ;
RUTTER, WJ .
BIOCHEMISTRY, 1979, 18 (24) :5294-5299
[7]   PANCREATIC CHOLESTEROL ESTERASES .1. PANCREATIC CHOLESTEROL ESTERASE INDUCTION DURING MATURATION [J].
COX, DG ;
LEUNG, CKT ;
KYGER, EM ;
SPILBURG, CA ;
LANGE, LG .
BIOCHEMISTRY, 1990, 29 (16) :3842-3848
[8]  
DIPERSIO LP, 1991, J BIOL CHEM, V266, P4033
[9]  
ERLSON C, 1975, SCAND J GASTROENTERO, V10, P401
[10]   EXPRESSION OF MEMBRANE INTERLEUKIN-1 BY FIBROBLASTS TRANSFECTED WITH MURINE PRO-INTERLEUKIN-1-ALPHA CDNA [J].
FUHLBRIGGE, RC ;
FINE, SM ;
UNANUE, ER ;
CHAPLIN, DD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (15) :5649-5653
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