CLEAVAGE OF HUMAN MDR1 MESSENGER-RNA BY A HAMMERHEAD RIBOZYME

被引：25

作者：

KOBAYASHI, H

DORAI, T

HOLLAND, JF

OHNUMA, T

机构：

[1] CUNY MT SINAI SCH MED,DEPT NEOPLAST DIS,1 GUSTAVE L LEVY PL,BOX 1128,NEW YORK,NY 10029

[2] CUNY MT SINAI SCH MED,DERALD H RUTTENBERG CANC CTR,NEW YORK,NY 10029

来源：

FEBS LETTERS | 1993年 / 319卷 / 1-2期

关键词：

HAMMERHEAD RIBOZYME; MULTIDRUG RESISTANCE;

D O I：

10.1016/0014-5793(93)80039-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We designed a hammerhead ribozyme which site-specifically cleaved the GUC sequence in codon 179 of MDR1 mRNA. The cleavage site was 6 amino acids upstream from the drug binding site and was considered sufficiently close to the essential locus for P-glycoprotein function. The ribozyme cleaved the MDR1 mRNA under physiological conditions in vitro. The cleavage was dependent on ribozyme concentration and on incubation time. Mg2+ ion was essential for the cleavage. These results show that a potentially useful tool is at hand which may inactivate MDR1 mRNA and revert the multidrug resistance phenotype.

引用

页码：71 / 74

页数：4

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