MIDKINE IS A MEDIATOR OF RETINOIC ACID-INDUCED NEURONAL DIFFERENTIATION OF EMBRYONAL CARCINOMA-CELLS

被引：46

作者：

MICHIKAWA, M

XU, RY

MURAMATSU, H

MURAMATSU, T

KIM, SU

机构：

[1] UNIV BRITISH COLUMBIA, DEPT MED, DIV NEUROL, VANCOUVER V6T 1W5, BC, CANADA

[2] KAGOSHIMA UNIV, FAC MED, DEPT BIOCHEM, KAGOSHIMA 890, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 192卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.1559

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Midkine (MK) is a novel growth factor and is the product of a retinoic acid responsive gene. When P19 murine embryonal carcinoma (EC) cells were exposed to MK, they differentiated into neurons, and the neuronal differentiation was accompanied by expression of choline acetyltransferase activity. Synthesis and release of MK in the EC cells treated with retinoic acid were shown by Western blot analysis, and rabbit anti-MK antibody attenuated the action of retinoic acid to induce the neuronal differentiation. These results indicate that MK is one of the mediators of retinoic acid action to induce the neuronal differentiation in EC cells. © 1993 Academic Press, Inc.

引用

页码：1312 / 1318

页数：7

共 29 条

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