RESCUING TRANSGENE EXPRESSION BY COINTEGRATION

被引：70

作者：

CLARK, AJ ^{[1
]}

COWPER, A ^{[1
]}

WALLACE, R ^{[1
]}

WRIGHT, G ^{[1
]}

SIMONS, JP ^{[1
]}

机构：

[1] PHARMACEUT PROT LTD,CTR GENOME RES,EDINBURGH EH9 3JQ,SCOTLAND

来源：

BIO-TECHNOLOGY | 1992年 / 10卷 / 11期

关键词：

D O I：

10.1038/nbt1192-1450

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

To test whether foreign gene expression can be improved in transgenic mice by manipulating the site of integration, we co-integrated the efficiently expressed sheep beta-lactoglobulin gene with two poorly expressed beta-lactoglobulin-derived hybrid genes encoding human proteins. In each case, we observed a significant improvement in the frequency and level of expression of the hybrid gene. ''Rescuing'' transgene expression by co-integration may provide a general solution for improving the efficiency of heterologous gene expression in transgenic animals.

引用

页码：1450 / 1454

页数：5

共 20 条

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