OSMOLAR CHANGES REGULATE THE PARACELLULAR PERMEABILITY OF CULTURED HUMAN CERVICAL EPITHELIUM

被引:21
作者
GORODESKI, GI
DESANTIS, BJ
GOLDFARB, J
UTIAN, WH
HOPFER, U
机构
[1] CASE WESTERN RESERVE UNIV, SCH MED, DEPT REPROD BIOL, CLEVELAND, OH 44106 USA
[2] CASE WESTERN RESERVE UNIV, SCH MED, DEPT PHYSIOL & BIOPHYS, CLEVELAND, OH 44106 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1995年 / 269卷 / 04期
关键词
TRANSEPITHELIAL ELECTRICAL CONDUCTANCE; TRANSEPITHELIAL ELECTRICAL RESISTANCE; TONICITY; NUCLEOTIDE RECEPTORS; EXTRACELLULAR ADENOSINE 5'-TRIPHOSPHATE;
D O I
10.1152/ajpcell.1995.269.4.C870
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Extracellular nucleotides induce a biphasic change in the transepithelial electrical conductance (G(T)) Of human cervical cells grown on filters: a rapid increase (phase I) followed by a sustained decrease (phase II). To probe the involvement of the intercellular space, its magnitude was varied by manipulating cell volume through changes in extracellular osmolarity. Under baseline conditions [G(T) = 115 mS/ cm(2) (similar to 9 Omega . cm(2))] and during phase II, hypertonic challenges resulted in an increase in G(T) (0.98%. mosmol(-1). l(-1) and 0.73%. mosmol(-1). l(-1), respectively). However, a hypertonic challenge during phase I decreased G(T) -(0.16%. mosmol(-1). l-1). Hypotonic challenges decreased GT during baseline, phase I, and phase II conditions by - 1%. mosmol(-1). l(-1). Similar trends were observed with regard to pyranine permeability. Reduction of extracellular calcium increased G(T), abrogated the phase II effect of extracellular ATP, and reversed the effect of a hypertonic challenge. The additive nature of the permeability changes in response to osmotic challenges and to ATP during phase II suggests that different sites are involved in each response, i.e., the resistance of the intercellular space changes with osmolarity and that of the tight junction during phase II.
引用
收藏
页码:C870 / C877
页数:8
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