OVEREXPRESSION OF C-MYC PRECEDES AMPLIFICATION OF THE GENE ENCODING DIHYDROFOLATE-REDUCTASE

被引：48

作者：

MAI, S

机构：

[1] Basel Institute for Immunology

来源：

GENE | 1994年 / 148卷 / 02期

关键词：

RECOMBINANT DNA; CHINESE HAMSTER OVARY CELLS; PROTOONCOGENE; ONCOPROTEIN; GENOMIC INSTABILITY; GENE AMPLIFICATION; FLUORESCENT IN SITU HYBRIDIZATION;

D O I：

10.1016/0378-1119(94)90696-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

An experimentally inducible model system was generated in which Chinese hamster ovary cells (CHO-9) were stably transfected with an inducible c-myc cDNA. The induction of c-myc in these transfectants is followed by the enhanced binding of c-Myc/Max-containing protein complexes to 5' flanking E-box sequences of the gene encoding dihydrofolate reductase (DHFR). Moreover, DHFR is transiently amplified. The inappropriate overproduction of the oncoprotein, therefore, seems to plays a role in induced DHFR amplification.

引用

页码：253 / 260

页数：8

共 48 条

[1]

AHNEN DJ, 1992, J CELL BIOCHEM, P143

[2] AMPLIFICATION OF CELLULAR ONCOGENES IN CANCER-CELLS [J].