学术探索
学术期刊
新闻热点
数据分析
智能评审

DETERMINATION OF ALPHA-AMYLASE ACTIVITY FROM COTTON LEAVES WITH AMYLOPECTIN-AZURE

被引：7
作者：
CHANG, CW
机构：
[1] Federal Research, Science and Education Administration, USDA, Phoenix
来源：
MICROCHEMICAL JOURNAL | 1979年 / 24卷 / 01期
关键词：
D O I：
10.1016/0026-265X(79)90036-5
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
α-Amylase activity from phenolic-rich plant tissues is successfully determined with an amylopectin-azure substrate. This procedure, due to the substrate's specificity for α-amylase, overcomes the effects of the interfering substances firmly associated with enzyme preparations. The procedure is more sensitive than the assay for activity with iodine solution. © 1979.
引用
收藏
页码：50 / 55
页数：6
相关论文
共 8 条
[1]   STARCH DEPLETION IN GERMINATING WHEAT, WRINKLED-SEEDED PEAS AND SENESCING TOBACCO-LEAVES [J].
ABBOTT, IR ;
MATHESON, NK .
PHYTOCHEMISTRY, 1972, 11 (04) :1261-&
[2]   ALPHA-AMYLASE IN GERMINATING DECORTICATED BARLEY .I.ALPHA-AMYLASE CONDITIONS OF GROWTH AND GRAIN CONSTITUENTS [J].
BRIGGS, DE .
PHYTOCHEMISTRY, 1968, 7 (04) :513-&
[3]   ACTIVATION-ENERGY FOR THERMAL INACTIVATION AND KM OF CARBONIC-ANHYDRASE FROM COTTON PLANT [J].
CHANG, CW .
PLANT SCIENCE LETTERS, 1975, 4 (02) :109-113
[4]   ENZYMIC DEGRADATION OF STARCH GRANULES IN COTYLEDONS OF GERMINATING PEAS [J].
JULIANO, BO ;
VARNER, JE .
PLANT PHYSIOLOGY, 1969, 44 (06) :886-&
[5]   EXTRACTION OF COTTON LEAF ENZYMES WITH BORATE [J].
KING, EE .
PHYTOCHEMISTRY, 1971, 10 (10) :2337-&
[6]   A NEW METHOD FOR DETERMINATION OF ALPHA-AMYLASE [J].
RINDERKNECHT, H ;
WILDING, P ;
HAVERBACK, BJ .
EXPERIENTIA, 1967, 23 (10) :805-+
[7]   CHANGES IN 3'-NUCLEOTIDASE DURING GERMINATION OF WHEAT EMBRYOS [J].
SHUSTER, L ;
GIFFORD, RH .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1962, 96 (03) :534-&
[8]  
1977, 1051 BIOR LAB TECHN
1