NITROUS-ACID MUTAGENESIS OF DUPLEX DNA AS A 3-COMPONENT SYSTEM

被引：24

作者：

THOMAS, HF ^{[1
]}

HARTMAN, PE ^{[1
]}

MUDRYJ, M ^{[1
]}

BROWN, DL ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,DEPT BIOL,BALTIMORE,MD 21218

来源：

MUTATION RESEARCH | 1979年 / 61卷 / 02期

关键词：

D O I：

10.1016/0027-5107(79)90121-0

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purified native Hemophilus influenzae DNA is relatively insusceptible to nitrous acid (NA) mutagenesis in vitro, but is readily mutated following denaturation. NA mutagenicity for duplex DNA is significantly increased in the presence of various alcohols, glycols, phenols or primary amines. Phenolextracted DNA contains dissociable contaminants of low molecular weight that enhance NA mutagenesis. Enhancement of NA mutagenesis by phenol and by spermine is due to the formation of unstable molecular species. We propose that reactive organic nitroso compounds are formed which then serve as delivery vehicles to promote mutagenicity of native DNA, perhaps via transnitrosation reactions. Similar reactions probably occur in vivo to promote NA-induced base substitution (but not frameshift) mutations in Salmonella typhimurium and in Escherichia coli. The possible significance of these observations to carcinogenesis is discussed. © 1979.

引用

页码：129 / 151

页数：23

共 92 条

[1] EFFECTS OF NITROUS ACID ON TRANSDUCTION BY SALMONELLA PHAGE P22 [J].