CYTOCHEMICAL-LOCALIZATION OF ENZYME MARKERS IN TRITRICHOMONAS-FETUS

被引：25

作者：

QUEIROZ, RCB ^{[1
]}

SANTOS, LMS ^{[1
]}

BENCHIMOL, M ^{[1
]}

DESOUZA, W ^{[1
]}

机构：

[1] UNIV FED RIO DE JANEIRO,INST BIOFIS CARLOS CHAGAS FILHO,DEPT PARASITOL & BIOFIS CELULAR,BR-21941 RIO DE JANEIRO,BRAZIL

来源：

PARASITOLOGY RESEARCH | 1991年 / 77卷 / 07期

关键词：

D O I：

10.1007/BF00931013

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Cytochemical techniques associated with transmission electron microscopy were used for the localization in Tritrichomonas foetus of enzymes used as markers of different cell structures. Reaction product indicating the presence of Mg2+-adenosine triphosphatase (Mg2+-ATPase) and 5'-nucleotidase was observed in the plasma membrane. Glucose-6-phosphatase was seen in association with the endoplasmic reticulum, revealing its organization as parallel cisternae. Thiaminopyrophosphatase was located in the cis-most region of the Golgi complex. Acid phosphatase was found within lysosomes as well as in several cisternae of the Golgi complex, in contrast to previous observations in mammalian cells. These observations provide support for the use of enzyme markers in future studies on cell fractionation of T. foetus.

引用

页码：561 / 566

页数：6

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