PCR-BASED ASSIGNMENT OF 2 MYOSIN HEAVY-CHAIN CDNA CLONES TO BIOCHEMICALLY AND HISTOCHEMICALLY DEFINED SINGLE TYPE-IIB AND TYPE-IID FIBERS OF RABBIT MUSCLE

被引：18

作者：

UBER, A ^{[1
]}

PETTE, D ^{[1
]}

机构：

[1] UNIV KONSTANZ,FAK BIOL,POSTFACH 5560 M641,D-78434 CONSTANCE,GERMANY

来源：

FEBS LETTERS | 1993年 / 331卷 / 1-2期

关键词：

FAST MYOSIN HEAVY CHAIN ISOFORM; MUSCLE FIBER TYPE; MYOSIN HEAVY CHAIN MESSENGER RNA ISOFORM; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SINGLE FIBER STUDY;

D O I：

10.1016/0014-5793(93)80324-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study assigns two as yet unidentified fast myosin cDNA clones to specific myosin heavy chain (MHC) isoforms and their mRNAs in different fiber types of rabbit skeletal muscle. Specific oligonucleotide primers were used for reverse transcription and polymerase chain reaction (PCR) to yield products of defined lengths. The method was sensitive enough to detect specific mRNA sequences in total RNA extracts from microdissected, freeze-dried, single-fiber fragments down to 16 ng dry weight. The fibers were typed histochemically and biochemically by their electrophoretically assessed MHC complement. The following results were obtained: clone pMHC20-40 was assigned to type IIB fibers and clone pMHC24-79 to type IID fibers.

引用

页码：193 / 197

页数：5

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