BIOTINYL ANALOGS OF AMYLIN AS BIOLOGICALLY-ACTIVE PROBES FOR AMYLIN CGRP RECEPTOR RECOGNITION

被引：3

作者：

CHANTRY, A

FOOT, EA

LEIGHTON, B

DAY, AJ

WILLIS, AC

机构：

[1] MRC Immunochemistry Unit, University of Oxford, Oxford, OX1 3QU, South Parks Road

[2] Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, South Parks Road

来源：

FEBS LETTERS | 1992年 / 296卷 / 02期

关键词：

AMYLIN; CGRP; RECEPTOR; BIOTINYLATION; NON-INSULIN-DEPENDENT DIABETES-MELLITUS;

D O I：

10.1016/0014-5793(92)80362-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Biotinyl analogues of rat amylin were synthesised with sulfosuccinimidyl 2-(biotinamido) ethyl-1,3-dithiopropionate (NHS-SS-Biotin). Biotinylated amylin peptides were purified by HPLC, quantitated, and the presence of the biotin group at Lys-1 confirmed by peroxidase-labelled avidin and FAB mass spectroscopy. Amylin-biotin retained a similar affinity for binding to rat liver plasma membranes compared with rat amylin an a so completely inhibited insulin-stimulated glycogen synthesis in rat soleus muscle incubated in vitro. These biologically active amylin probes will enable a complete analysis of amylin/CGRP receptor expression in various cell types and facilitate the isolation and characterisation of the hormone-receptor complex.

引用

页码：123 / 127

页数：5

共 16 条

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