HERPES-SIMPLEX VIRUS-1 RNA-BINDING PROTEIN-US11 NEGATIVELY REGULATES THE ACCUMULATION OF A TRUNCATED VIRAL MESSENGER-RNA

The U(s)11 gene of herpes simplex virus 1 (HSV-1) encodes a site-specific, basic, RNA-binding protein. The viral RNA sequences bound by U(s)11 protein precipitated by a monoclonal antibody hybridized to a 1.3-kb BamHI C' fragment of the HSV-1 genome. This fragment encodes a U(s)11-regulated transcript which accumulates to high level in the cells infected with U(s)11- virus but not in cells infected with wild-type virus. This transcript, designated DELTA-34, is a truncated form of the mRNA encoding an essential protein encoded by the U(L)34 open reading frame. The U(s)11 protein was shown to bind DELTA-34 RNA at or near its 3' terminus. The nucleotide sequence of the region surrounding the termination of transcription of DELTA-34 RNA transcription suggests that the latter may be the product of transcriptional attenuation. U(s)11 protein resembles the tat protein of human immunodeficiency virus with respect to size, charge, nucleolar accumulation, and possibly effect on accumulation of its target RNA but does not share with it discernible sequence homology.