THE EFFECT OF PROGESTERONE UPON 1ST TRIMESTER TROPHOBLASTIC CELL-DIFFERENTIATION AND HUMAN CHORIONIC-GONADOTROPIN SECRETION

被引：16

作者：

BARNEA, ER ^{[1
]}

FELDMAN, D ^{[1
]}

KAPLAN, M ^{[1
]}

机构：

[1] UNIV MED & DENT NEW JERSEY,ROBERT WOOD JOHNSON MED CTR,DEPT OBSTET GYNECOL,CAMDEN,NJ

来源：

HUMAN REPRODUCTION | 1991年 / 6卷 / 07期

关键词：

CELLS; CULTURE; HCG; PROGESTERONE; SUPERFUSION;

D O I：

10.1093/oxfordjournals.humrep.a137456

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The effect of progesterone (P) upon first trimester placental secretion of human chorionic gonadotrophin (HCG) and cellular differentiation was studied using both static and kinetic methods. At 1-mu-M, P inhibited spontaneous episodic secretion of HCG when given in short pulses (1-4 min) to placental explants in superfusion. Both HCG pulse frequency and amplitude were reduced. At 0.1-0.01-mu-M P concentrations, the effect of HCG secretion was milder. P also blocked the maximally effective concentration 100 pM of gonadotrophin releasing hormone (GnRH) analogue, a known HCG stimulant, when given together with it for 1 min. This inhibitory effect lasted for 1 h after P administration. Progesterone at 1-mu-M, added daily for 1 week blocked HCG secretion by isolated trophoblastic cells in static culture. This inhibitory effect lasted until the fifth day. No effect on differentiation and long-term viability was noticed in P-treated cells. Incubation with 0.1-1.0-mu-M P did not affect HCG secretion by explants after 24 h. In contrast, the effect of 1-mu-M cortisol or 1 nM oestradiol was stimulatory. In conclusion, P exerts both a rapid and delayed inhibitory effect upon HCG secretion and production. It may do so by counteracting the stimulatory effect of endogenous GnRH on gonadotrophin secretion by the placenta.

引用

页码：905 / 909

页数：5

共 23 条

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