AMPEROMETRIC SENSOR BASED ON THE ALKALINE-PHOSPHATASE ACTIVITY

被引：16

作者：

PARIENTE, F ^{[1
]}

HERNANDEZ, L ^{[1
]}

LORENZO, E ^{[1
]}

机构：

[1] UNIV AUTONOMA MADRID,FAC CIENCIAS,DEPT QUIM ANALIT & ANAL INSTRUMENTAL,E-28049 MADRID,SPAIN

来源：

BIOELECTROCHEMISTRY AND BIOENERGETICS | 1992年 / 27卷 / 01期

关键词：

D O I：

10.1016/0302-4598(92)85014-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The construction and response of an immobilized enzyme electrode as an amperometric sensor are described. Alkaline phosphatase was covalently bonded to a nylon filter mesh using glutaraldehyde and bovine serum albumin (BSA) as crosslinkers. This modified membrane was then attached to the surface of a glassy-carbon (GC) electrode. Substrate mass transport and enzymatic catalysis control were investigated in a rotating disk electrode. Various response characteristics and kinetic parameters were evaluated and are compared to those of a previously reported amperometric alkaline phosphatase electrode.

引用

页码：73 / 87

页数：15

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