GAMMA-INTERFERON COUNTERACTS INTERLEUKIN-1-ALPHA STIMULATED EXPRESSION OF UROKINASE-TYPE PLASMINOGEN-ACTIVATOR IN HUMAN ENDOTHELIAL-CELLS INVITRO

被引：30

作者：

WOJTA, J ^{[1
]}

ZOELLNER, H ^{[1
]}

GALLICCHIO, M ^{[1
]}

HAMILTON, JA ^{[1
]}

MCGRATH, K ^{[1
]}

机构：

[1] UNIV MELBOURNE,DEPT MED,PARKVILLE,VIC 3050,AUSTRALIA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 188卷 / 01期

关键词：

D O I：

10.1016/0006-291X(92)92407-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of γ-interferon (γ-IFN) on the interleukin-1α (IL-1γ) induced stimulation of urokinasetype plasminogen activator (u-PA) expression in human foreskin microvascular endothelial cells (HFMEC) and in human umbilical vein endothelial cells (HFMEC) was investigated. When γ-IFN and IL-1α were added to the cells simultaneously, γ-IFN inhibited the IL-1α induced increase in uPA antigen production in both HFMEC and HUVEC in a dose dependent fashion, with a maximum inhibitory effect achieved between 2.0 and 20.0U/ml of γ-IFN. Pretreatment of HFMEC with γ-IFN for 1 hour before addition of IL-1α resulted in a significant reduction in u-PA synthesis. However, when HFMEC were pretreated for 8 hours with γ-IFN before the addition of IL-1α the reduction in u-PA production was even more significant. When γ-IFN was added to HFMEC 1 hour after IL-1α, a significant inhibition in u-PA synthesis was seen. In contrast only a slight inhibition in IL-1α induced u-PA production was seen when γ-IFN was added to the cells 8 hours after IL-1a. γ-IFN also inhibited significantly the IL-1α induced increase in u-PA specific mRNA in HUVEC and HFMEC. © 1992.

引用

页码：463 / 469

页数：7

共 24 条

[1] UROKINASE-TYPE PLASMINOGEN-ACTIVATOR - PROENZYME, RECEPTOR, AND INHIBITORS [J].